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Dr Margaret Hunt
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REAL TIME PCR TUTORIAL SPREADSHEET FOR PFAFFL CALCULATIONS |
| HTML version prepared by Dr Richard Hunt and Srilata Kukunuri |
 Excel
spread sheet for calculation of Pfaffl equationIf you get a password box, click cancel. The Excel file should then load. The Excel file (link at logo above left) includes the formulae that we use (row 3) and also a description of what the formulae are (row 8) since the results rather than the formulae show when you print an Excel file. We do the calculation one step at a time so that it is easier to see how the calculations are done and also because we routinely like to scan for things such as whether the control gene is changing appreciably - and that is what column J in the attached file will tell you. |
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This page copyright
2005-2006, The Board of
Trustees of the University of South Carolina
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